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In vitro attachment and symmetry breaking of a human embryo model assembled from primed embryonic stem cells

Cornell Affiliated Author(s)

Author

M. Simunovic
E.D. Siggia
A.H. Brivanlou

Abstract

Our knowledge of the molecular mechanisms surrounding human embryo implantation and gastrulation is lacking, largely due to technical and ethical limitations of experimenting with human embryos. Alternatives to human embryos have been reported, in which 3D clusters of embryonic stem cells are differentiated in a stepwise manner to model aspects of human embryogenesis. Yet it remains challenging to model the events past attachment. We propose a strategy of modeling the post-attachment human embryo by assembling a pre-formed polarized epithelial epiblast and extraembryonic cells, allowing them to self-organize into a structure that mimics the dish-attached human embryo. The model attaches in vitro and, in the absence of exogenous morphogens, breaks anteroposterior symmetry, giving rise to early gastrulation cell types. Our assembloid approach enables in a modular way to upgrade or exchange extraembryonic tissues to access more advanced stages of post-attachment development while complying with ethical policies. © 2022 Elsevier Inc.

Date Published

Journal

Cell Stem Cell

Volume

29

Issue

6

Number of Pages

962-972.e4,

URL

https://www.scopus.com/inward/record.uri?eid=2-s2.0-85131116982&doi=10.1016%2fj.stem.2022.05.001&partnerID=40&md5=08637bb8df4c2437d12d3f9a1acf04de

DOI

10.1016/j.stem.2022.05.001

Research Area

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