High-Resolution Protein Structure Determination by Serial Femtosecond Crystallography
Abstract
Structure determination of proteins and other macromolecules has historically required the growth of high-quality crystals sufficiently large to diffract x-rays efficiently while withstanding radiation damage. We applied serial femtosecond crystallography (SFX) using an x-ray free-electron laser (XFEL) to obtain high-resolution structural information from microcrystals (less than 1 micrometer by 1 micrometer by 3 micrometers) of the well-characterized model protein lysozyme. The agreement with synchrotron data demonstrates the immediate relevance of SFX for analyzing the structure of the large group of difficult-to-crystallize molecules.
Date Published
Journal
Science
Volume
337
Issue
6092
Number of Pages
362-364,
URL
https://www.scopus.com/inward/record.uri?eid=2-s2.0-84864004802&doi=10.1126%2fscience.1217737&partnerID=40&md5=9778b7e924b92b04bb0a9a47e1048c09
DOI
10.1126/science.1217737
Research Area
Group (Lab)
Sol M. Gruner Group